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Catalog
C001519
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Alternative name
C-C motif chemokine 20 CLIA KIT; Beta-chemokine exodus-1 CLIA KIT; CC chemokine LARC CLIA KIT; Liver and activation-regulated chemokine CLIA KIT; Macrophage inflammatory protein 3 alpha CLIA KIT; MIP-3-alphaCCL20 CLIA KIT; LARC CLIA KIT; MIP3A CLIA KIT; SCYA20 CLIA KIT; MIP-3-alpha CLIA KIT
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GeneMIP3a
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Species ReactivityRat
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a).
No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range1.37-1000pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Inflammatory Protein 3 Alpha (MIP3a) level in the sample or standard.
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