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Catalog
C001518
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Alternative name
Macrophage migration inhibitory factor CLIA KIT; Glycosylation-inhibiting factor CLIA KIT; GIF CLIA KIT; L-dopachrome isomerase CLIA KIT; L-dopachrome tautomerase (EC:5.3.3.12) CLIA KIT; Phenylpyruvate tautomeraseMIF CLIA KIT; GLIF CLIA KIT; MMIF CLIA KIT; MIF CLIA KIT; GIF CLIA KIT
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GeneMIF
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Other Species
Human MIF clia kitRat MIF clia kitMouse MIF clia kit
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Migration Inhibitory Factor (MIF).
No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 6.4pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Migration Inhibitory Factor (MIF) level in the sample or standard.
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