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Catalog
C001460
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Alternative name
Matrix metalloproteinase-9 CLIA KIT; 92 kDa gelatinase CLIA KIT; 92 kDa type IV collagenase CLIA KIT; Gelatinase B CLIA KIT; GELBMMP9 CLIA KIT; MMP-9 CLIA KIT; GELB CLIA KIT
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GeneMMP9
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Other Species
Mouse MMP9 clia kit
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of MMP9. No significant cross-reactivity or interference between MMP9 and analogues was observed.
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Detection Range41.2-30,000pg/mL. The standard curve concentrations used for the CLIA's were 30,000pg/mL, 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL, 41.2pg/mL.
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SensitivityThe minimum detectable dose of MMP9 is typically less than 15.7pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MMP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the MMP9 level in the sample or standard.
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