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Catalog
C001409
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Alternative name
Macrophage migration inhibitory factor CLIA KIT; Glycosylation-inhibiting factor CLIA KIT; GIF CLIA KIT; L-dopachrome isomerase CLIA KIT; L-dopachrome tautomerase (EC:5.3.3.12) CLIA KIT; Phenylpyruvate tautomeraseMIF CLIA KIT; GLIF CLIA KIT; MMIF CLIA KIT; MIF CLIA KIT; GIF CLIA KIT
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GeneMIF
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Species ReactivityMouse
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Detection Range15.625~1000pg/mL
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Sensitivity9.375pg/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIF. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MIF. You can calculate the concentration of Mouse MIF in the samples by comparing the RLU value of the samples to the standard curve.
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