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Catalog
C001280
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Alternative name
Myoglobin CLIA KIT; MB CLIA KIT;
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GeneMYO
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Other Species
Mouse MYO clia kitRat MYO clia kitHuman MYO clia kitMouse MYO clia kitHuman MYO clia kitRat MYO clia kit
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Species ReactivityPorcine
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SpecificityThis assay has high sensitivity and excellent specificity for detection of myoglobin. No significant cross-reactivity or interference between myoglobin and analogues was observed.
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Detection Range0.27-200ng/mL. The standard curve concentrations used for the CLIA's were 200ng/mL, 66.67ng/mL, 22.22ng/mL, 7.41ng/mL, 2.47ng/mL, 0.82ng/mL, 0.27ng/mL.
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SensitivityThe minimum detectable dose of myoglobin is typically less than 0.10ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to myoglobin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to myoglobin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the myoglobin level in the sample or standard.
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