SpecificityThis assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF).
No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.
SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Intended UseHuman NGF clia kit allows for the in vitro quantitative determination of NGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nerve Growth Factor (NGF) level in the sample or standard.
No manual or datasheet available for this product.