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Catalog
C001228
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Alternative name
Neutrophil gelatinase-associated lipocalin CLIA KIT; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 CLIA KIT; Lipocalin-2 CLIA KIT; Oncogene 24p3 CLIA KIT; Siderocalin LCN2 CLIA KIT; p25LCN2 CLIA KIT; HNL CLIA KIT; NGAL CLIA KIT; NGAL CLIA KIT
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GeneNGAL
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Other Species
Rat NGAL clia kitMouse NGAL clia kitHuman NGAL clia kitHuman NGAL clia kit
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL).
No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.
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SamplesSerum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Detection Range27.43-20000pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Gelatinase Associated Lipocalin (NGAL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Gelatinase Associated Lipocalin (NGAL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil Gelatinase Associated Lipocalin (NGAL) level in the sample or standard.
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