SpecificityThis assay has high sensitivity and excellent specificity for detection of NLRP3. No significant cross-reactivity or interference between NLRP3 and analogues was observed.
SamplesHuman tissue homogenates, cell lysates and other biological fluids.
Sensitivity27.5pg/mL.
Intended UseHuman NLRP3 clia kit allows for the in vitro quantitative determination of NLRP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of NLRP3 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLRP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the NLRP3 level in the sample or standard.
No manual or datasheet available for this product.