SpecificityThis kit recognizes natural and recombinant NT-proBNP. No significant cross-reactivity or interference between NT-proBNP and analogues was observed.
SamplesSerum, plasma and other biological fluids.
Sensitivity9.375pg/mL
Intended UseHuman NT-proBNP clia kit allows for the in vitro quantitative determination of NT-proBNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of NT-proBNP concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to NT-proBNP. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of NT-proBNP. You can calculate the concentration of NT-proBNP in the samples by comparing the RLU value of the samples to the standard curve.
No manual or datasheet available for this product.