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Catalog
C000115
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Alternative name
Vascular endothelial growth factor C CLIA KIT; Flt4 ligand CLIA KIT; Flt4-L CLIA KIT; Vascular endothelial growth factor-related protein CLIA KIT; VRPVEGFC CLIA KIT; VEGF-C CLIA KIT; Flt4-L CLIA KIT; VRP CLIA KIT
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GeneVEGFC
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of VEGFC. No significant cross-reactivity or interference between VEGFC and analogues was observed.
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Detection Range2.74-2,000pg/mL. The standard curve concentrations used for the CLIA's were 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, 8.23pg/mL, 2.74pg/mL.
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SensitivityThe minimum detectable dose of VEGFC is typically less than 0.92pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGFC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the VEGFC level in the sample or standard.
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