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Catalog
C001148
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Alternative name
Nuclear factor interleukin-3-regulated protein CLIA KIT; E4 promoter-binding protein 4 CLIA KIT; Interleukin-3 promoter transcriptional activator CLIA KIT; Interleukin-3-binding protein 1 CLIA KIT; Transcriptional activator NF-IL3ANFIL3 CLIA KIT; E4BP4 CLIA KIT; IL3BP1 CLIA KIT
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GeneNFIL3
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Other Species
NFIL3 clia kit NFIL3 clia kitMouse NFIL3 clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Nuclear Factor, Interleukin 3 Regulated (NFIL3)
No significant cross-reactivity or interference between Nuclear Factor, Interleukin 3 Regulated (NFIL3) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor, Interleukin 3 Regulated (NFIL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor, Interleukin 3 Regulated (NFIL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nuclear Factor, Interleukin 3 Regulated (NFIL3) level in the sample or standard.
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