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Catalog
C001101
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GeneOVA
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Other Species
Human OVA sIgE clia kitMouse OVA sIgE clia kit
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Species ReactivityAll
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA).
No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.
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SamplesTissue homogenates, cell lysates and other biological fluids
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Detection Range1.17-300ug/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ovalbumin (OVA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Ovalbumin (OVA) and unlabeled Ovalbumin (OVA) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ovalbumin (OVA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Ovalbumin (OVA) level in the sample or standard.
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