SpecificityThis assay has high sensitivity and excellent specificity for detection of OxLDL. No significant cross-reactivity or interference between OxLDL and analogues was observed.
SamplesMouse serum, plasma and other biological fluids.
Detection Range1.37-1,000pg/mL. The standard curve concentrations used for the CLIA's were 1,000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL, 4.12pg/mL, 1.37pg/mL.
Product Description specificalIntended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of OxLDL in mouse serum, plasma and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to OxLDL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to OxLDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the OxLDL level in the sample or standard.