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Catalog
C001013
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GenePDAP1
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Other Species
PDAP1 clia kit PDAP1 clia kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of PDGFA Associated Protein 1 (PDAP1)
No significant cross-reactivity or interference between PDGFA Associated Protein 1 (PDAP1) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range6.859-5000pg/mL The standard curve concentrations used for the ELISA's were 5000pg/mL, 1666.67pg/mL, 555.56pg/mL, 185.19pg/mL, 61.728pg/mL, 20.576pg/mL, 6.859pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 31pg/mL
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDGFA Associated Protein 1 (PDAP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDGFA Associated Protein 1 (PDAP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalThe microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the PDGFA Associated Protein 1 (PDAP1) level in the sample or standard.
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